10/27/2023 0 Comments Gene construction kit 2 file![]() ![]() The Tol2kit system uses site-specific recombination-based cloning (multisite Gateway technology) to allow quick, modular assembly of - constructs in a Tol2 transposon backbone. In zebrafish, transgenesis has suffered from three problems: the labor of building complex expression constructs using conventional subcloning low transgenesis efficiency, leading to mosaicism in transient transgenics and infrequent germline incorporation and difficulty in identifying germline integrations unless using a fluorescent marker transgene. Transgenesis is an important tool for assessing gene function.
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